Seminar on 07/06/2010 (room 3/40, building 34, monday 12:15) under the title ,, Image processing and analysis as a supporting tool for investigations of the living cells and stained tissue sections “ will be delivered by dr Anna Korzyńska (Institute of Biocybernetics and Biomedical Engeneering Polish Academy of Sciences). The lecture concerns some problems and solutions for application of the image processing methodology to the quantitative analysis of chosen types of microscopic images. Immunohistochemically stained images of thin tissue sections and time-laps microscopy images from bright field microscopes are considered. Presented method concerns the segmentation problems in colour and grey scale images. The features of an image used in the presented method are colour, texture, the strongest gradients location and the model of cell shape. Whilst the proposed methodology is based on mathematical morphology operations, including watershed, adaptive and context dependent thresholdings, Robert’s gradient detectors, Gaussian pyramid and, in the case of colour images on artificial colour space adjusted to main colours in image, but in the case of grey scale images on the supporting images from various positions in Z-axis or fluorescent images from GFP transfected cells.